Shimadzu Spd20a User Manual
Tares the current absorbance output to 0.0000 AU. Use this after the baseline has stabilized with the mobile phase flowing. [LAMP]: Turns the Deuterium ( D2cap D sub 2 ) or Tungsten ( ) lamps ON or OFF manually. [ENTER]: Confirms value entries and menu selections. 4. Standard Operating Procedure (SOP) Startup Sequence Turn on the main power switch of the SPD-20A. Press the [LAMP] key to ignite the D2cap D sub 2
| Symptom | Probable Cause | Corrective Action | |---------|----------------|--------------------| | High baseline noise | Air bubbles in cell | Flush with degassed mobile phase or remove cell and rinse. | | Negative peaks | Sample absorbs less than mobile phase at set wavelength | Change wavelength or check mobile phase UV cutoff. | | Lamp fails to ignite | Lamp expired or power supply issue | Check lamp hours; replace D2 lamp. | | No signal at high sensitivity | Flow cell window dirty | Clean cell with 1M HNO₃, then water, then methanol. | | Wavelength error | Stepper motor failure or filter stuck | Run Wavelength Calibration from service menu (qualified personnel only). |
The is a high-performance, dual-wavelength UV/Vis detector designed for use with Shimadzu’s Prominence and LC-20A series liquid chromatography systems. This user manual serves as the definitive technical guide for laboratory personnel responsible for installation, operation, maintenance, and troubleshooting of the detector. shimadzu spd20a user manual
Turn off the detector, unplug the power cord, and wait at least 30 minutes for the lamp to cool down completely. The lamp gets hot enough to cause severe burns.
Periodic cleaning of the cell keeps baseline noise minimal, crucial for trace analysis. 5. Troubleshooting the SPD-20A Tares the current absorbance output to 0
Before maintenance, disconnect the power cable at the rear of the unit.
Shimadzu SPD-20A/20AV UV-VIS Manual | PDF | Mains Electricity [ENTER]: Confirms value entries and menu selections
Before operating the SPD-20A, users must ensure it is installed correctly to prevent hardware damage or safety hazards. The user manual emphasizes:
Always turn the power OFF and disconnect the power cable at the rear of the instrument before cleaning, maintenance, or replacing lamps.
Disclaimer: This article is a summary based on common knowledge and provided search results. Always consult the specific, official Shimadzu manual provided with your instrument for the most accurate information.
Place on a stable surface, away from direct sunlight and temperature fluctuations.
